Hotspot

trnF-GAA-ndhJ

LSC · 51770-52476 · 707 bp

Back to Macleaya

Pi 0.0128

MarkerSeek score 66.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 9
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	844-848 bp	1.0	81.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	808-812 bp	1.0	80.8
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	845-849 bp	1.0	80.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	809-813 bp	1.0	80.2
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	57.9	58.0	832-836 bp	1.0	80.1