Hotspot

psaA-ycf3

LSC · 45109-45757 · 649 bp

Back to Macleaya

Pi 0.0123

MarkerSeek score 65.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 8
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	760-762 bp	1.0	78.4
psaA-ycf3_p2	GAGAAATGACCTGGTCTGGC	TTGGTTGAAGATCACGAGGC	55.0	50.0	58.3	58.2	778-780 bp	1.0	77.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	801-803 bp	1.0	77.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGGTTTGAATAA	50.0	45.0	57.9	57.5	749-751 bp	1.0	76.3
psaA-ycf3_p5	GAGAAATGACCTGGTCTGGC	GCTATAGCGCTTACTCCAGG	55.0	55.0	58.3	57.6	819-821 bp	1.0	75.9