Hotspot

rps16-trnQ-UUG

LSC · 6330-8247 · 1918 bp

Back to Macleaya

Pi 0.0105
MarkerSeek score 64.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1918
variable_sites
20
pi
0.0105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2002-2009 bp 1.0 80.4
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2000-2007 bp 1.0 80.4
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2008-2015 bp 1.0 80.3
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 2009-2016 bp 1.0 80.3
rps16-trnQ-UUG_p5 GTCGAGCTAAGAGCACCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 2055-2062 bp 1.0 80.0