primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TAAAGACGGTTTTCGGTGCT	GGTCGTGCAATCCAATCTCT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.273440	1831	4396	2971-2986	1.000000	78.9
trnK-UUU_p2	trnK-UUU	2	GGACTCCCAAGCACACAAAT	GGTCGTGCAATCCAATCTCT	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.409016	1831	4396	2856-2871	1.000000	77.8
trnK-UUU_p3	trnK-UUU	3	ATATGCCCGTGTCAACCAAT	GGTCGTGCAATCCAATCTCT	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.465211	1831	4396	2778-2793	1.000000	77.4
trnK-UUU_p4	trnK-UUU	4	ATGCCCGTGTCAACCAATAT	GGTCGTGCAATCCAATCTCT	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.465211	1831	4396	2776-2791	1.000000	77.4
trnK-UUU_p5	trnK-UUU	5	ATGCCCGTGTCAACCAATAT	ACAAGAGGCCCCCTATGAAT	20	20	45.0	50.0	57.6	58.1	0.0	0.0	0.522510	1831	4396	2954-2971	1.000000	77.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.104521	6330	8247	2002-2009	1.000000	80.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.105939	6330	8247	2000-2007	1.000000	80.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	6330	8247	2008-2015	1.000000	80.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GAATCCACAGCCGATCATGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.117059	6330	8247	2009-2016	1.000000	80.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GTCGAGCTAAGAGCACCTTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.1	58.1	0.6	16.9	0.160149	6330	8247	2055-2062	1.000000	80.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	20	20	45.0	45.0	58.0	56.4	0.0	0.0	1.674622	9633	10579	1041-1059	1.000000	69.9
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AGGAAACGGAAAGAGAGGGA	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.662051	9633	10579	1095-1113	1.000000	69.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	58.2	56.4	0.0	0.0	1.787764	9633	10579	1090-1108	1.000000	68.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GAGGAAACGGAAAGAGAGGG	AACCGAAAGACCCCTTAACT	20	20	55.0	45.0	57.6	56.4	0.0	0.0	2.024726	9633	10579	1096-1114	1.000000	66.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	20	20	50.0	45.0	57.6	56.4	0.9	0.0	2.046364	9633	10579	1027-1045	1.000000	66.9
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	11338	11477	208-220	1.000000	68.5
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	11338	11477	211-223	1.000000	68.2
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	11338	11477	214-226	1.000000	67.8
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	11338	11477	217-229	1.000000	67.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	11338	11477	212-224	1.000000	66.0
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.413468	30701	31884	1243-1339	1.000000	73.4
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.598551	30701	31884	1249-1345	1.000000	71.9
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30701	31884	1189-1285	1.000000	70.6
petN-psbM_p4	petN-psbM	4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	20	20	45.0	45.0	58.0	56.1	0.0	0.0	1.936016	30701	31884	1243-1339	1.000000	69.2
petN-psbM_p5	petN-psbM	5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	57.8	56.1	0.0	0.0	2.121100	30701	31884	1249-1345	1.000000	67.7
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	TTCATCGGATCCTAGGTCGG	ATGGATTCTTCCTGGGTCGA	20	20	55.0	50.0	58.4	58.1	10.8	0.0	0.503103	33237	33631	567-583	1.000000	79.3
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	TTCATCGGATCCTAGGTCGG	TGGATTCTTCCTGGGTCGAT	20	20	55.0	50.0	58.4	58.1	10.8	0.0	0.503103	33237	33631	566-582	1.000000	79.3
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	ATGGATTCTTCCTGGGTCGA	20	20	50.0	50.0	58.5	58.1	7.1	0.0	0.597757	33237	33631	506-522	1.000000	79.0
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	TGGATTCTTCCTGGGTCGAT	20	20	50.0	50.0	58.5	58.1	7.1	0.0	0.597757	33237	33631	505-521	1.000000	79.0
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	TTCATCGGATCCTAGGTCGG	TGGCAATATGTCTACGCTGG	20	20	55.0	50.0	58.4	57.8	10.8	0.0	0.621620	33237	33631	510-526	1.000000	78.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33848	34394	611-650	1.000000	83.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	33848	34394	590-629	1.000000	83.3
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.366926	33848	34394	576-615	1.000000	82.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.385076	33848	34394	555-594	1.000000	82.6
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGAAAAAGCCCCTTATCGG	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.401198	33848	34394	635-674	1.000000	82.3
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AATTGAATCAAACGGGCCCT	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.1	58.2	22.4	0.0	0.308879	34467	36070	1778-1783	1.000000	78.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	ATTGAATCAAACGGGCCCTT	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.1	58.2	22.4	0.0	0.308879	34467	36070	1777-1782	1.000000	78.9
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	AATTGAATCAAACGGGCCCT	GACCGGACCAACCTACAAAA	20	20	45.0	50.0	58.1	57.7	22.4	9.5	0.322075	34467	36070	1792-1797	1.000000	78.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	ATTGAATCAAACGGGCCCTT	GACCGGACCAACCTACAAAA	20	20	45.0	50.0	58.1	57.7	22.4	9.5	0.322075	34467	36070	1791-1796	1.000000	78.8
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	22.4	0.0	0.340432	34467	36070	1768-1773	1.000000	78.7
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	45109	45757	760-762	1.000000	78.4
psaA-ycf3_p2	psaA-ycf3	2	GAGAAATGACCTGGTCTGGC	TTGGTTGAAGATCACGAGGC	20	20	55.0	50.0	58.3	58.2	26.0	0.0	0.447593	45109	45757	778-780	1.000000	77.2
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCAGG	20	20	50.0	55.0	57.9	57.6	4.1	18.7	0.471060	45109	45757	801-803	1.000000	77.0
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGGTTTGAATAA	20	20	50.0	45.0	57.9	57.5	4.1	0.0	0.574403	45109	45757	749-751	1.000000	76.3
psaA-ycf3_p5	psaA-ycf3	5	GAGAAATGACCTGGTCTGGC	GCTATAGCGCTTACTCCAGG	20	20	55.0	55.0	58.3	57.6	26.0	18.7	0.611721	45109	45757	819-821	1.000000	75.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	50013	50722	750-760	1.000000	81.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	50013	50722	758-768	1.000000	80.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	50013	50722	782-792	1.000000	80.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	50013	50722	790-800	1.000000	79.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	50013	50722	773-783	1.000000	79.3
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.015623	51770	52476	844-848	1.000000	81.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.065013	51770	52476	808-812	1.000000	80.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	51770	52476	845-849	1.000000	80.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	51770	52476	809-813	1.000000	80.2
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CTGGTAGAGCAGAGGACTGA	GTTGGATAGGATGGCCCTTG	20	20	55.0	55.0	57.9	58.0	2.5	0.0	0.149416	51770	52476	832-836	1.000000	80.1
petA-psbJ_p1	petA-psbJ	1	GGACGCCAAGTGGTTGATAT	AACTGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043011	67863	68983	1957-1959	1.000000	80.8
petA-psbJ_p2	petA-psbJ	2	GGACGCCAAGTGGTTGATAT	ACTGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043011	67863	68983	1956-1958	1.000000	80.8
petA-psbJ_p3	petA-psbJ	3	GGACGCCAAGTGGTTGATAT	ACACAATCAAACCCGAACGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.082090	67863	68983	1743-1745	1.000000	80.6
petA-psbJ_p4	petA-psbJ	4	GGACGCCAAGTGGTTGATAT	GGACTAGCTGTACCTACCGT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.088275	67863	68983	1839-1841	1.000000	80.5
petA-psbJ_p5	petA-psbJ	5	GGACGCCAAGTGGTTGATAT	GAGCCGACAAGGAATTCCAT	20	20	50.0	50.0	58.0	58.0	0.0	26.2	0.082510	67863	68983	1981-1983	1.000000	80.4
ndhF_p1	ndhF	1	TCCACCTAGTTTGTCAGCTT	TGACCAATTGTAACTTCTTGA	20	21	45.0	33.3	56.4	53.0	0.0	4.4	7.630980	116189	118567	2476-2567	1.000000	40.7
ndhF_p2	ndhF	2	TCCACCTAGTTTGTCAGCTT	TTGACCAATTGTAACTTCTTGA	20	22	45.0	31.8	56.4	53.9	0.0	4.4	7.751563	116189	118567	2477-2568	1.000000	40.7
ndhF_p3	ndhF	3	TCCACCTAGTTTGTCAGCTT	TGATTTGATCATTTGACCAATTGT	20	24	45.0	29.2	56.4	55.8	0.0	4.4	7.797833	116189	118567	2489-2580	1.000000	40.7
ndhF_p4	ndhF	4	TCCACCTAGTTTGTCAGCTT	TCATTTGACCAATTGTAACTTCTTGA	20	26	45.0	30.8	56.4	57.8	0.0	4.4	7.850532	116189	118567	2481-2572	1.000000	40.7
ndhF_p5	ndhF	5	TCCACCTAGTTTGTCAGCTT	TGACCAATTGTAACTTCTTGATTTGA	20	26	45.0	30.8	56.4	57.8	0.0	4.4	7.850532	116189	118567	2476-2567	1.000000	40.7
ccsA-ndhD_p1	ccsA-ndhD	1	AGGGCTACATAGTTATGGTTCA	TCCGGATTTCGTTCTCTCAC	22	20	40.9	50.0	56.3	57.4	4.3	0.0	4.324545	121858	122118	374-384	1.000000	50.0
ccsA-ndhD_p2	ccsA-ndhD	2	AGGGCTACATAGTTATGGTTCA	CCGGATTTCGTTCTCTCACT	22	20	40.9	50.0	56.3	57.3	4.3	0.0	4.331774	121858	122118	373-383	1.000000	50.0
ccsA-ndhD_p3	ccsA-ndhD	3	AGGGCTACATAGTTATGGTTCAT	TCCGGATTTCGTTCTCTCAC	23	20	39.1	50.0	56.8	57.4	4.3	0.0	4.822522	121858	122118	374-384	1.000000	46.1
ccsA-ndhD_p4	ccsA-ndhD	4	AGGGCTACATAGTTATGGTTCAT	CCGGATTTCGTTCTCTCACT	23	20	39.1	50.0	56.8	57.3	4.3	0.0	4.829751	121858	122118	373-383	1.000000	46.0
ccsA-ndhD_p5	ccsA-ndhD	5	AGGGCTACATAGTTATGGTTCA	ATCCGGATTTCGTTCTCTCAC	22	21	40.9	47.6	56.3	57.8	4.3	12.4	4.841703	121858	122118	375-385	1.000000	45.9
ycf1_p1	ycf1	1	ACCGCAGTTCCTTATGTTGT	TGTCGAGAAAGAGGAAATGCT	20	21	45.0	42.9	57.4	57.3	0.0	0.0	2.246617	130800	130919	240-240	1.000000	63.7
ycf1_p2	ycf1	2	ACCGCAGTTCCTTATGTTGT	TCGAGAAAGAGGAAATGCTGA	20	21	45.0	42.9	57.4	57.0	0.0	0.0	2.522958	130800	130919	238-238	1.000000	61.5
ycf1_p3	ycf1	3	TGACCGCAGTTCCTTATGTT	TGTCGAGAAAGAGGAAATGCT	20	21	45.0	42.9	57.1	57.3	0.0	0.0	2.536402	130800	130919	242-242	1.000000	61.4
ycf1_p4	ycf1	4	TTGACCGCAGTTCCTTATGT	TGTCGAGAAAGAGGAAATGCT	20	21	45.0	42.9	57.1	57.3	0.0	0.0	2.536402	130800	130919	243-243	1.000000	61.4
ycf1_p5	ycf1	5	ACCGCAGTTCCTTATGTTGT	GTCGAGAAAGAGGAAATGCTG	20	21	45.0	47.6	57.4	57.0	0.0	0.0	2.568367	130800	130919	239-239	1.000000	61.1
rps19-rpl22_p1	rps19-rpl22	1	TGGGTAGAGCCGGATCTAAG	CGCTTTCTCCATTAGCCCAT	20	20	55.0	50.0	58.0	58.0	29.9	0.0	0.030330	161743	161801	1104-1104	0.500000	59.8
rps19-rpl22_p2	rps19-rpl22	2	CCACAATGATCGGCCATACA	CGCTTTCTCCATTAGCCCAT	20	20	50.0	50.0	58.0	58.0	2.2	0.0	0.061193	161743	161801	698-698	0.500000	59.5
rps19-rpl22_p3	rps19-rpl22	3	TGGGTAGAGCCGGATCTAAG	CGTTGACTTCGGCTTGACTA	20	20	55.0	50.0	58.0	57.9	29.9	0.0	0.063527	161743	161801	873-873	0.500000	59.5
rps19-rpl22_p4	rps19-rpl22	4	TGGGTAGAGCCGGATCTAAG	ATTCCCTCGACACCTGAAGA	20	20	55.0	50.0	58.0	58.1	29.9	0.0	0.064617	161743	161801	1314-1314	0.500000	59.5
rps19-rpl22_p5	rps19-rpl22	5	TGGGTAGAGCCGGATCTAAG	TTCCCTCGACACCTGAAGAT	20	20	55.0	50.0	58.0	58.1	29.9	0.0	0.064617	161743	161801	1313-1313	0.500000	59.5
rpl22_p1	rpl22	1	TGGGTAGAGCCGGATCTAAG	TTCCTCGTGGTTTACCCTCT	20	20	55.0	50.0	58.0	58.0	29.9	3.0	0.023009	161802	162332	1409-1409	0.500000	59.8
rpl22_p2	rpl22	2	CCACAATGATCGGCCATACA	TTCCTCGTGGTTTACCCTCT	20	20	50.0	50.0	58.0	58.0	2.2	3.0	0.053873	161802	162332	1003-1003	0.500000	59.6
rpl22_p3	rpl22	3	TGGGTAGAGCCGGATCTAAG	CCATTATTCCTCGTCCGCAA	20	20	55.0	50.0	58.0	58.1	29.9	0.0	0.055097	161802	162332	1795-1795	0.500000	59.6
rpl22_p4	rpl22	4	TGGGTAGAGCCGGATCTAAG	CCTCGTCCGCAAATATCCAA	20	20	55.0	50.0	58.0	58.1	29.9	0.0	0.055097	161802	162332	1787-1787	0.500000	59.6
rpl22_p5	rpl22	5	TCCCGGGCATCTACCATTAT	TTCCTCGTGGTTTACCCTCT	20	20	50.0	50.0	58.0	58.0	18.5	3.0	0.058670	161802	162332	1025-1025	0.500000	59.5