Hotspot

ycf1

SSC · 128463-134027 · 5565 bp

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Pi 0.00145

MarkerSeek score 45.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 45
pi: 0.00145
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTCGCGATATTGAAGTAGA	AAGAAGGGATTGATGAAACA	40.9	35.0	57.7	52.3	203 bp	1.0	42.4
ycf1_p2	TGCTTCGCGATATTGAAGTAGA	AGAAGGGATTGATGAAACAA	40.9	35.0	57.7	52.3	202 bp	1.0	42.4
ycf1_p3	TGCTTCGCGATATTGAAGTAGA	AAAGAAGGGATTGATGAAACA	40.9	33.3	57.7	53.3	204 bp	1.0	42.4
ycf1_p4	TGCTTCGCGATATTGAAGTAGA	AAGAAGGGATTGATGAAACAA	40.9	33.3	57.7	53.3	203 bp	1.0	42.4
ycf1_p5	TGCTTCGCGATATTGAAGTAGA	AGAAGGGATTGATGAAACAAA	40.9	33.3	57.7	53.3	202 bp	1.0	42.4