Hotspot

ccsA

SSC · 118947-119897 · 951 bp

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Pi 0.000769

MarkerSeek score 32.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 951
variable_sites: 2
pi: 0.000769
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATCTTCTAAA	TCCTATGTATTCTTCAGGCCCT	50.0	45.5	58.0	58.1	1074-1075 bp	1.0	63.6
ccsA_p2	GAGTGGCGGCATCTTCTAAA	TGTATTCTTCAGGCCCTTTCT	50.0	42.9	58.0	56.6	1069-1070 bp	1.0	61.1
ccsA_p3	GAGTGGCGGCATCTTCTAAA	TCCTATGTATTCTTCAGGCCC	50.0	47.6	58.0	56.6	1074-1075 bp	1.0	60.7
ccsA_p4	GAGTGGCGGCATCTTCTAAA	CCTATGTATTCTTCAGGCCCT	50.0	47.6	58.0	56.6	1073-1074 bp	1.0	60.6
ccsA_p5	AGTGGCGGCATCTTCTAAAA	TCCTATGTATTCTTCAGGCCCT	45.0	45.5	57.5	58.1	1073-1074 bp	1.0	59.9