Hotspot

ndhF-rpl32

SSC · 115991-117219 · 1229 bp

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Pi 0.00337

MarkerSeek score 55.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1229
variable_sites: 24
pi: 0.00337
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1246-1341 bp	1.0	83.1
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1240-1335 bp	1.0	83.1
ndhF-rpl32_p3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.3	57.7	1241-1336 bp	1.0	81.2
ndhF-rpl32_p4	AACAAGCAGAAGTCCCAACC	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.3	57.7	1244-1339 bp	1.0	80.8
ndhF-rpl32_p5	GGAACAAGCAGAAGTCCCAA	TACGTTTCTTTGGAACCGCC	50.0	50.0	58.0	58.8	1245-1340 bp	1.0	79.4