Hotspot

rpl2-rpl23

LSC · 89335-89363 · 29 bp

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Pi 0.0139

MarkerSeek score 53.70

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 29
variable_sites: 2
pi: 0.0139
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TCGCGGATTGGATTTCACTT	CGCTTCAACCGGGTTATTCT	45.0	50.0	57.9	58.3	169-173 bp	1.0	78.9
rpl2-rpl23_p2	TTCGCGGATTGGATTTCACT	CGCTTCAACCGGGTTATTCT	45.0	50.0	57.9	58.3	170-174 bp	1.0	78.9
rpl2-rpl23_p3	CGCGGATTGGATTTCACTTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	168-172 bp	1.0	78.3
rpl2-rpl23_p4	TCGCGGATTGGATTTCACTT	ATCATTACGCTTCAACCGGG	45.0	50.0	57.9	58.3	176-180 bp	1.0	78.2
rpl2-rpl23_p5	TTCGCGGATTGGATTTCACT	ATCATTACGCTTCAACCGGG	45.0	50.0	57.9	58.3	177-181 bp	1.0	78.2