Hotspot

accD-psaI

LSC · 61874-62458 · 585 bp

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Pi 0.00217

MarkerSeek score 47.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 7
pi: 0.00217
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTACACGGTTTCTTTCCC	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	730-793 bp	1.0	77.8
accD-psaI_p2	GCTACACGGTTTCTTTCCCT	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	729-792 bp	1.0	77.8
accD-psaI_p3	AGCTACACGGTTTCTTTCCC	AGAAGCCATTGCAATTGCTG	50.0	45.0	57.8	57.6	581-734 bp	1.0	76.3
accD-psaI_p4	GCTACACGGTTTCTTTCCCT	AGAAGCCATTGCAATTGCTG	50.0	45.0	57.8	57.6	580-733 bp	1.0	76.3
accD-psaI_p5	GCTACACGGTTTCTTTCCCT	TTGCTGGAAACACTAAGCCC	50.0	50.0	57.8	58.4	719-782 bp	1.0	76.3