Hotspot

ycf3-trnS-GGA

LSC · 46665-47476 · 812 bp

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Pi 0.00325
MarkerSeek score 48.40
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
812
variable_sites
10
pi
0.00325
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT TTGTGGTCGGTTGGTGAATT 50.0 45.0 58.1 57.9 1017-1026 bp 1.0 80.6
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.1 58.2 987-996 bp 1.0 79.8
ycf3-trnS-GGA_p3 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.1 57.8 937-946 bp 1.0 79.6
ycf3-trnS-GGA_p4 TCTCCTGAGGTTGTCGGAAT ATTGTGGTCGGTTGGTGAAT 50.0 45.0 58.1 57.7 1018-1027 bp 1.0 78.8
ycf3-trnS-GGA_p5 TTCTCCTGAGGTTGTCGGAA TTGTGGTCGGTTGGTGAATT 50.0 45.0 58.3 57.9 1018-1027 bp 1.0 78.8