Hotspot

atpA-atpF

LSC · 12968-13037 · 70 bp

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Pi 0.00298

MarkerSeek score 47.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 2
pi: 0.00298
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	GCCTACCTGAAGTACTGTGC	CGGGGCCATGAAAGAAGTAA	55.0	50.0	58.0	57.9	202-207 bp	1.0	81.3
atpA-atpF_p2	GCCTACCTGAAGTACTGTGC	TAATATTGGCATGCTCGGGG	55.0	50.0	58.0	57.8	217-222 bp	1.0	80.5
atpA-atpF_p3	TCGCCTACCTGAAGTACTGT	CGGGGCCATGAAAGAAGTAA	50.0	50.0	57.8	57.9	204-209 bp	1.0	79.6
atpA-atpF_p4	GCCTACCTGAAGTACTGTGC	CGGTGCTAATATTGGCATGC	55.0	50.0	58.0	57.7	223-228 bp	1.0	79.3
atpA-atpF_p5	CTACCTGAAGTACTGTGCCG	CGGGGCCATGAAAGAAGTAA	55.0	50.0	57.7	57.9	200-205 bp	1.0	79.1