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trnK-UUU-rps16

LSC · 4374-5194 · 821 bp

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Pi 0.00147

MarkerSeek score 48.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 7
pi: 0.00147
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	949-955 bp	1.0	79.5
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	948-954 bp	1.0	79.5
trnK-UUU-rps16_p3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	45.0	45.0	57.8	58.0	970-976 bp	1.0	79.2
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	958-964 bp	1.0	78.5
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	952-958 bp	1.0	77.8