Hotspot

trnH-GUG-psbA

LSC · 97-492 · 396 bp

Back to Machilus

Pi 0.00784

MarkerSeek score 55.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 13
pi: 0.00784
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	444-467 bp	1.0	84.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	458-481 bp	1.0	84.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	457-480 bp	1.0	84.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	459-482 bp	1.0	84.3
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	473-496 bp	1.0	81.4