Hotspot

ndhE

SSC · 122757-123059 · 303 bp

Back to Macadamia

Pi 0.0099

MarkerSeek score 45.30

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 3
pi: 0.0099
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GCGAGTTGTCTCATGCCATA	CCATTTTGTAGCTGCTGCAC	50.0	50.0	58.1	58.0	1469-1471 bp	1.0	79.8
ndhE_p2	GCAGAGGCAATTTGCTTAGC	CCATTTTGTAGCTGCTGCAC	50.0	50.0	58.1	58.0	1369-1371 bp	1.0	79.7
ndhE_p3	GCGAGTTGTCTCATGCCATA	GGGTCACTTCACTGGTTTGT	50.0	50.0	58.1	57.9	1341-1343 bp	1.0	79.6
ndhE_p4	GTTGGACAGGCAGATTCACA	CCATTTTGTAGCTGCTGCAC	50.0	50.0	58.1	58.0	1423-1425 bp	1.0	79.5
ndhE_p5	TGTTGGACAGGCAGATTCAC	CCATTTTGTAGCTGCTGCAC	50.0	50.0	58.1	58.0	1424-1426 bp	1.0	79.5