Hotspot

ndhD

SSC · 120562-122067 · 1506 bp

Back to Macadamia

Pi 0.00199

MarkerSeek score 48.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 3
pi: 0.00199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACTACGTTTAAAAATGCCAGT	TATTTCGAGTACGGCTTTTT	33.3	35.0	54.0	53.0	1629-1632 bp	1.0	40.3
ndhD_p2	TCTACTACGTTTAAAAATGCCAGT	TATTTCGAGTACGGCTTTTT	33.3	35.0	56.6	53.0	1632-1635 bp	1.0	40.3
ndhD_p3	CTACTACGTTTAAAAATGCCAGT	TATTTCGAGTACGGCTTTTT	34.8	35.0	55.3	53.0	1631-1634 bp	1.0	40.3
ndhD_p4	TTCTACTACGTTTAAAAATGCCAGT	TATTTCGAGTACGGCTTTTT	32.0	35.0	57.3	53.0	1633-1636 bp	1.0	40.3
ndhD_p5	ACTACGTTTAAAAATGCCAGT	TCTGTTCAAAGCATATCTTGT	33.3	33.3	54.0	53.1	1610 bp	1.0	40.2