Hotspot

ndhD-psaC

SSC · 122068-122199 · 132 bp

Back to Macadamia

Pi 0.0775

MarkerSeek score 86.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 132
variable_sites: 10
pi: 0.0775
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TAAAGAACCCGCGGATATCG	TATGGCATGAGACAACTCGC	50.0	50.0	57.6	58.1	230-233 bp	1.0	80.6
ndhD-psaC_p2	TAAAGAACCCGCGGATATCG	GTGAATCTGCCTGTCCAACA	50.0	50.0	57.6	58.1	275-278 bp	1.0	79.5
ndhD-psaC_p3	TAAAGAACCCGCGGATATCG	TGTGAATCTGCCTGTCCAAC	50.0	50.0	57.6	58.1	276-279 bp	1.0	79.5
ndhD-psaC_p4	TAAAGAACCCGCGGATATCG	TGAATCTGCCTGTCCAACAG	50.0	50.0	57.6	57.8	274-277 bp	1.0	78.8
ndhD-psaC_p5	TAAAGAACCCGCGGATATCG	GATGTGAATCTGCCTGTCCA	50.0	50.0	57.6	57.6	278-281 bp	1.0	77.0