Hotspot

petD-rpoA

LSC · 81579-81751 · 173 bp

Back to Macadamia

Pi 0.0173

MarkerSeek score 62.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 3
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTACCGCAGTAGCTCTTTG	TGGAAATCAAAGATCTTGGCCT	55.0	40.9	58.4	57.9	358-360 bp	1.0	61.4
petD-rpoA_p2	GTACCGCAGTAGCTCTTTGG	TGGAAATCAAAGATCTTGGCCT	55.0	40.9	58.4	57.9	357-359 bp	1.0	61.4
petD-rpoA_p3	CCGCAGTAGCTCTTTGGTTA	TGGAAATCAAAGATCTTGGCCT	50.0	40.9	57.6	57.9	354-356 bp	1.0	61.2
petD-rpoA_p4	GGTACCGCAGTAGCTCTTTG	TGGACCTTTTGAACAAGAGTGA	55.0	40.9	58.4	57.8	391-393 bp	1.0	59.9
petD-rpoA_p5	GTACCGCAGTAGCTCTTTGG	TGGACCTTTTGAACAAGAGTGA	55.0	40.9	58.4	57.8	390-392 bp	1.0	59.9