Hotspot

petB-petD

LSC · 80112-81014 · 903 bp

Back to Macadamia

Pi 0.00443

MarkerSeek score 61.30

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 4
pi: 0.00443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AGCAGGGTATTTCTGGTCCT	ATGACCCATCCCTTTAGCCA	50.0	50.0	58.0	58.4	1043 bp	1.0	76.9
petB-petD_p2	AGCAGGGTATTTCTGGTCCT	AGGGATCCATCCTCCAAAGG	50.0	55.0	58.0	58.5	959 bp	1.0	76.3
petB-petD_p3	AGCAGGGTATTTCTGGTCCT	TGACCCATCCCTTTAGCCAA	50.0	50.0	58.0	58.6	1042 bp	1.0	75.1
petB-petD_p4	AGCAGGGTATTTCTGGTCCT	GACCCATCCCTTTAGCCAAT	50.0	50.0	58.0	57.2	1041 bp	1.0	74.0
petB-petD_p5	AGCAGGGTATTTCTGGTCCT	GACCCATCCCTTTAGCCAATT	50.0	47.6	58.0	58.0	1041 bp	1.0	71.8