Hotspot

psaJ-rpl33

LSC · 70705-71069 · 365 bp

Back to Macadamia

Pi 0.00551

MarkerSeek score 44.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 365
variable_sites: 2
pi: 0.00551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGTTTATTTCCGGATGCGT	CTGACATCTTTACCCTTAGCCA	45.0	45.5	58.0	57.5	434-438 bp	1.0	60.7
psaJ-rpl33_p2	CGTGGCACCTGTGTTAAGTA	CTGACATCTTTACCCTTAGCCA	50.0	45.5	57.8	57.5	497-501 bp	1.0	59.5
psaJ-rpl33_p3	TCGTTTATTTCCGGATGCGT	TGACATCTTTACCCTTAGCCA	45.0	42.9	58.0	56.3	433-437 bp	1.0	59.4
psaJ-rpl33_p4	ATTTCCGGATGCGTTGACAT	CTGACATCTTTACCCTTAGCCA	45.0	45.5	58.2	57.5	428-432 bp	1.0	58.8
psaJ-rpl33_p5	TCGTTTATTTCCGGATGCGT	TCTGACATCTTTACCCTTAGCC	45.0	45.5	58.0	57.2	435-439 bp	1.0	58.6