Hotspot

ycf4-cemA

LSC · 63580-64474 · 895 bp

Back to Macadamia

Pi 0.00337

MarkerSeek score 61.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 895
variable_sites: 3
pi: 0.00337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	ATGCAAGATATGGGAGGGGA	45.0	50.0	57.9	57.9	961-964 bp	1.0	78.8
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	ATGCAAGATATGGGAGGGGA	50.0	50.0	58.1	57.9	963-966 bp	1.0	78.5
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	ATGCAAGATATGGGAGGGGA	45.0	50.0	57.8	57.9	988-991 bp	1.0	78.1
ycf4-cemA_p4	TGCGCGTACCAATTGAAGTA	GGAGGGGAGTGAATGCTTTC	45.0	55.0	57.9	58.2	949-952 bp	1.0	78.0
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	ATGCAAGATATGGGAGGGGA	45.0	50.0	57.8	57.9	986-989 bp	1.0	77.7