Hotspot

accD-psaI

LSC · 61793-62504 · 712 bp

Back to Macadamia

Pi 0.00423

MarkerSeek score 61.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 3
pi: 0.00423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	837-839 bp	1.0	77.2
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	836-838 bp	1.0	77.2
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AGAAGCCATTGCAATTGCTG	50.0	45.0	57.8	57.6	841-843 bp	1.0	75.7
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AGAAGCCATTGCAATTGCTG	50.0	45.0	57.8	57.6	840-842 bp	1.0	75.7
accD-psaI_p5	GCTCCATGGTTTCTTTCCCT	GAAGCCATTGCAATTGCTGG	50.0	50.0	57.8	58.6	839-841 bp	1.0	73.6