Hotspot

rbcL-accD

LSC · 59555-60289 · 735 bp

Back to Macadamia

Pi 0.00544

MarkerSeek score 61.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 4
pi: 0.00544
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCTCGCGAAGGTAATGAAA	CACTTCCGCTGGTATTTCCA	45.0	50.0	57.9	57.9	965 bp	1.0	78.8
rbcL-accD_p2	TTGCTCGCGAAGGTAATGAA	CACTTCCGCTGGTATTTCCA	45.0	50.0	57.9	57.9	966 bp	1.0	78.8
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	CACTTCCGCTGGTATTTCCA	50.0	50.0	57.9	57.9	907 bp	1.0	78.6
rbcL-accD_p4	GCAAATGGAGCCCTGAACTA	CACTTCCGCTGGTATTTCCA	50.0	50.0	57.9	57.9	930 bp	1.0	78.5
rbcL-accD_p5	TAGCAAATGGAGCCCTGAAC	CACTTCCGCTGGTATTTCCA	50.0	50.0	57.9	57.9	932 bp	1.0	78.4