Hotspot

psbC-trnS-UGA

LSC · 38972-39182 · 211 bp

Back to Macadamia

Pi 0.0286
MarkerSeek score 69.10
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
211
variable_sites
6
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 333 bp 1.0 80.2
psbC-trnS-UGA_p2 CTTTGTGGGTCATTTGTGGC GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.8 58.0 415 bp 1.0 80.0
psbC-trnS-UGA_p3 CTTTGTGGGTCATTTGTGGC GCTGAGTGGTTGATAGCTCC 50.0 55.0 57.8 58.1 407 bp 1.0 79.7
psbC-trnS-UGA_p4 CTTTGTGGGTCATTTGTGGC GGCTGAGTGGTTGATAGCTC 50.0 55.0 57.8 58.1 408 bp 1.0 79.7
psbC-trnS-UGA_p5 CTTTGTGGGTCATTTGTGGC GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.8 58.1 400 bp 1.0 79.7