Hotspot

petN-psbM

LSC · 30979-32192 · 1214 bp

Back to Macadamia

Pi 0.00577

MarkerSeek score 62.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1214
variable_sites: 7
pi: 0.00577
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTTCGCTCGTAGTATGGGG	TGCTACTGCACTCTTCGTTC	55.0	50.0	57.9	57.9	1329 bp	1.0	78.8
petN-psbM_p2	TTCGCTCGTAGTATGGGGAA	TGCTACTGCACTCTTCGTTC	50.0	50.0	58.2	57.9	1327 bp	1.0	78.0
petN-psbM_p3	TTTCGCTCGTAGTATGGGGA	TGCTACTGCACTCTTCGTTC	50.0	50.0	58.2	57.9	1328 bp	1.0	78.0
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCGTTC	50.0	50.0	57.6	57.9	1315 bp	1.0	77.1
petN-psbM_p5	CTTTCGCTCGTAGTATGGGG	GCTACTGCACTCTTCGTTCT	55.0	50.0	57.9	57.6	1328 bp	1.0	76.5