Hotspot

trnS-GCU-trnG-UCC

LSC · 9556-10525 · 970 bp

Back to Macadamia

Pi 0.0177

MarkerSeek score 68.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 17
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	TCGAGAGGGGAAGGATGAAA	50.0	50.0	58.2	57.8	1161-1166 bp	1.0	78.8
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	CATCGAGAGGGGAAGGATGA	50.0	55.0	58.2	58.3	1163-1168 bp	1.0	78.5
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TCATCGAGAGGGGAAGGATG	50.0	55.0	58.2	58.3	1164-1169 bp	1.0	78.5
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	ATCGAGAGGGGAAGGATGAA	50.0	50.0	58.2	57.5	1162-1167 bp	1.0	77.0
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ATCATCGAGAGGGGAAGGAT	50.0	50.0	58.2	57.3	1165-1170 bp	1.0	75.1