Hotspot

rps16

LSC · 56607-57667 · 1061 bp

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Pi 0

MarkerSeek score 61.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1061
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACCGGATCCAAACTAAAGGA	AGAAGTCATCCACACCCTCT	45.0	50.0	56.1	57.7	998-1118 bp	1.0	66.5
rps16_p2	ACCGGATCCAAACTAAAGGAA	AGAAGTCATCCACACCCTCT	42.9	50.0	56.9	57.7	998-1118 bp	1.0	64.7
rps16_p3	ACCGGATCCAAACTAAAGGAAT	AGAAGTCATCCACACCCTCT	40.9	50.0	57.4	57.7	998-1118 bp	1.0	60.6
rps16_p4	ACCGGATCCAAACTAAAGGA	GAAGTCATCCACACCCTCTT	45.0	50.0	56.1	56.8	997-1117 bp	1.0	59.8
rps16_p5	ACCGGATCCAAACTAAAGGA	GGTGTCAATAGAACAAGGGGG	45.0	52.4	56.1	58.3	1058-1179 bp	1.0	58.9