Hotspot

psaC

SSC · 117289-117534 · 246 bp

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Pi 0

MarkerSeek score 21.00

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TGAGAACAGGTTTTTGACAGT	TGATGTTCAAAATTTACTATAGATCCA	38.1	25.9	55.6	55.2	329 bp	1.0	40.0
psaC_p2	TTGAGAACAGGTTTTTGACAGT	TGATGTTCAAAATTTACTATAGATCCA	36.4	25.9	56.3	55.2	330 bp	1.0	40.0
psaC_p3	TGAGAACAGGTTTTTGACAGT	GATGTTCAAAATTTACTATAGATCCA	38.1	26.9	55.6	53.7	328 bp	1.0	40.0
psaC_p4	TGAGAACAGGTTTTTGACAGT	TGATGTTCAAAATTTACTATAGATCC	38.1	26.9	55.6	53.7	329 bp	1.0	40.0
psaC_p5	TTTGAGAACAGGTTTTTGACAGT	TGATGTTCAAAATTTACTATAGATCCA	34.8	25.9	57.0	55.2	331 bp	1.0	40.0