Hotspot

atpI

LSC · 26221-26964 · 744 bp

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Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 TTTTTAGAGGTCAGGGCAAT ACACTGAATCGTAAAAAGGGA 40.0 38.1 54.5 55.2 801 bp 1.0 40.1
atpI_p2 TTTTTAGAGGTCAGGGCAAT ACACTGAATCGTAAAAAGGGAT 40.0 36.4 54.5 55.7 801 bp 1.0 40.1
atpI_p3 TTTTTAGAGGTCAGGGCAAT ACACTGAATCGTAAAAAGGG 40.0 40.0 54.5 53.5 801 bp 1.0 40.1
atpI_p4 TTTTTAGAGGTCAGGGCAAT ACACTGAATCGTAAAAAGGGATT 40.0 34.8 54.5 56.4 801 bp 1.0 40.1
atpI_p5 TTTTTAGAGGTCAGGGCAAT CACTGAATCGTAAAAAGGGA 40.0 40.0 54.5 53.2 800 bp 1.0 40.1