Hotspot

ndhF-rpl32

SSC · 112918-113731 · 814 bp

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Pi 0
MarkerSeek score 59.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
814
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAGTAGAAGACCCGCTCCT TCAATGCTGTCCAATACCCC 50.0 50.0 58.1 57.6 979-987 bp 1.0 76.8
ndhF-rpl32_p2 AAAGTAGAAGACCCGCTCCT CAATGCTGTCCAATACCCCC 50.0 55.0 58.1 58.6 978-986 bp 1.0 75.5
ndhF-rpl32_p3 AAAAGTAGAAGACCCGCTCC TCAATGCTGTCCAATACCCC 50.0 50.0 57.2 57.6 980-988 bp 1.0 71.1
ndhF-rpl32_p4 AAGTAGAAGACCCGCTCCTA TCAATGCTGTCCAATACCCC 50.0 50.0 57.2 57.6 978-986 bp 1.0 70.7
ndhF-rpl32_p5 AAAGTAGAAGACCCGCTCCT TTCAATGCTGTCCAATACCCC 50.0 47.6 58.1 58.3 980-988 bp 1.0 70.1