Hotspot

psbK-trnQ-UUG

LSC · 55524-56068 · 545 bp

Back to Maackia

Pi 0.00367

MarkerSeek score 59.70

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 2
pi: 0.00367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	TAGGCTTCGGGTAATTTCGC	ATATTCTGCTCTGGGACGGA	50.0	50.0	58.1	57.9	716-718 bp	1.0	79.1
psbK-trnQ-UUG_p2	TAGGCTTCGGGTAATTTCGC	TATTCTGCTCTGGGACGGAA	50.0	50.0	58.1	58.1	715-717 bp	1.0	78.7
psbK-trnQ-UUG_p3	GCGTAGGCTTCGGGTAATTT	ATATTCTGCTCTGGGACGGA	50.0	50.0	58.3	57.9	719-721 bp	1.0	76.8
psbK-trnQ-UUG_p4	GCGTAGGCTTCGGGTAATTT	TATTCTGCTCTGGGACGGAA	50.0	50.0	58.3	58.1	718-720 bp	1.0	76.4
psbK-trnQ-UUG_p5	TAGGCTTCGGGTAATTTCGC	GATATTCTGCTCTGGGACGG	50.0	55.0	58.1	57.6	717-719 bp	1.0	76.2