Hotspot

psbZ-trnG-GCC

LSC · 45616-46009 · 394 bp

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Pi 0

MarkerSeek score 31.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	551 bp	1.0	77.0
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	549 bp	1.0	76.6
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	AAAAAGGGTAGGGGCGGATA	50.0	50.0	57.8	58.4	587 bp	1.0	75.2
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	AAAAAGGGTAGGGGCGGATA	55.0	50.0	58.3	58.4	585 bp	1.0	74.7
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	TACACAAAAAGGGTAGGGGC	50.0	50.0	57.8	57.4	592 bp	1.0	73.6