Hotspot

trnS-UGA-psbZ

LSC · 45043-45426 · 384 bp

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Pi 0

MarkerSeek score 51.70

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CGTCGACCAAAAGGAGAGAG	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	583-587 bp	1.0	78.8
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	500-504 bp	1.0	78.8
trnS-UGA-psbZ_p3	GAGCTATCAACCACTCAGCC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	507-511 bp	1.0	78.6
trnS-UGA-psbZ_p4	GGAGCTATCAACCACTCAGC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	508-512 bp	1.0	78.6
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	515-519 bp	1.0	78.5