Hotspot

psbC-trnS-UGA

LSC · 44742-44949 · 208 bp

Back to Maackia

Pi 0.0577

MarkerSeek score 82.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 12
pi: 0.0577
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCATTTATGGCACGCAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.9	58.0	403 bp	1.0	81.2
psbC-trnS-UGA_p2	TCATTTATGGCACGCAGGAA	GCTGAGTGGTTGATAGCTCC	45.0	55.0	57.9	58.1	395 bp	1.0	81.0
psbC-trnS-UGA_p3	TCATTTATGGCACGCAGGAA	GGCTGAGTGGTTGATAGCTC	45.0	55.0	57.9	58.1	396 bp	1.0	81.0
psbC-trnS-UGA_p4	TCATTTATGGCACGCAGGAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.9	58.1	388 bp	1.0	81.0
psbC-trnS-UGA_p5	TCATTTATGGCACGCAGGAA	GCTTTTGGAGAGATGGCTGA	45.0	50.0	57.9	57.9	410 bp	1.0	80.4