Hotspot

petN-psbM

LSC · 37374-38675 · 1302 bp

Back to Maackia

Pi 0.00538

MarkerSeek score 62.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1302
variable_sites: 7
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AAACGGTAAGTCAAAGCGAC	45.0	45.0	58.0	56.7	1400-1401 bp	1.0	70.0
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1456-1457 bp	1.0	69.2
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	AAAACGGTAAGTCAAAGCGAC	45.0	42.9	58.0	57.4	1401-1402 bp	1.0	67.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AAACGGTAAGTCAAAGCGAC	50.0	45.0	57.6	56.7	1346-1347 bp	1.0	67.2
petN-psbM_p5	CCCTCGTAGTATGGGGAAGA	AAACGGTAAGTCAAAGCGAC	55.0	45.0	57.6	56.7	1356-1357 bp	1.0	67.0