Hotspot

rbcL-atpB

LSC · 6586-7312 · 727 bp

Back to Maackia

Pi 0.00138
MarkerSeek score 59.80
Primer available yes
Label rbcL-atpB

Feature Metadata

label_name
rbcL-atpB
feature_type
igs
region
LSC
length_bp
727
variable_sites
1
pi
0.00138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-atpB_p1 AACACCAGCTTTGAACCCAA CCTGGGGGAAAAGCTACATC 45.0 55.0 58.1 57.9 889-891 bp 1.0 78.7
rbcL-atpB_p2 AACACCAGCTTTGAACCCAA CTGGGGGAAAAGCTACATCC 45.0 55.0 58.1 57.9 888-890 bp 1.0 78.7
rbcL-atpB_p3 AACACCAGCTTTGAACCCAA GGGGGAAAAGCTACATCCAG 45.0 55.0 58.1 57.9 886-888 bp 1.0 78.7
rbcL-atpB_p4 AACACCAGCTTTGAACCCAA GGACCGATTATTTGGGCGAT 45.0 50.0 58.1 58.1 862-864 bp 1.0 78.3
rbcL-atpB_p5 AACACCAGCTTTGAACCCAA AAGCTACATCCAGTACCGGA 45.0 50.0 58.1 57.8 879-881 bp 1.0 77.9