Hotspot

rps15-ycf1

SSC · 123407-123787 · 381 bp

Back to Lysionotus

Pi 0.0225

MarkerSeek score 48.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 381
variable_sites: 30
pi: 0.0225
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	AACAGCAAACAAGTTTCGGC	50.0	45.0	58.0	58.1	771-786 bp	1.0	84.0
rps15-ycf1_p2	AGTCGTTGACGTTTTCCCAA	AACAGCAAACAAGTTTCGGC	45.0	45.0	58.0	58.1	767-782 bp	1.0	83.8
rps15-ycf1_p3	TCCCAACGCGTTACATAAGG	AACAGCAAACAAGTTTCGGC	50.0	45.0	58.0	58.1	1162-1177 bp	1.0	83.6
rps15-ycf1_p4	AACACCATGCATTGATGGGT	AACAGCAAACAAGTTTCGGC	45.0	45.0	58.1	58.1	1025-1040 bp	1.0	83.6
rps15-ycf1_p5	CAGCAGTCGTTGACGTTTTC	GCACAAAGTTCTGCCTGTTC	50.0	50.0	58.0	57.9	910-916 bp	0.791667	73.3