Hotspot

ndhD-psaC

SSC · 117145-117244 · 100 bp

Back to Lysionotus

Pi 0.0374

MarkerSeek score 61.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 100
variable_sites: 11
pi: 0.0374
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGATTCGTGGTAAAGACAAGA	CCGACCGATTTCTTGAGTGT	38.1	50.0	54.9	57.9	193-198 bp	1.0	54.4
ndhD-psaC_p2	TGATTCGTGGTAAAGACAAGA	AGCGATGTGAATCTGCATGT	38.1	45.0	54.9	58.0	213-218 bp	1.0	54.0
ndhD-psaC_p3	TGATTCGTGGTAAAGACAAGA	TGTGAATCTGCATGTCCGAC	38.1	50.0	54.9	58.3	208-213 bp	1.0	52.3
ndhD-psaC_p4	GGAAAATGATTCGTGGTAAAGACA	CCGACCGATTTCTTGAGTGT	37.5	50.0	57.8	57.9	199-204 bp	0.958333	51.2
ndhD-psaC_p5	GGAAAATGATTCGTGGTAAAGACA	AGCGATGTGAATCTGCATGT	37.5	45.0	57.8	58.0	219-224 bp	0.958333	50.9