Hotspot

ccsA-ndhD

SSC · 115383-115623 · 241 bp

Back to Lysionotus

Pi 0.0354
MarkerSeek score 74.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
241
variable_sites
30
pi
0.0354
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CAATTCAGCAATTGTGGCGT TCAGTTGAAAAGGTTGAAGT 45.0 35.0 57.9 52.8 405-429 bp 1.0 49.7
ccsA-ndhD_p2 CAATTCAGCAATTGTGGCGT TCAGTTGAAAAGGTTGAAGTT 45.0 33.3 57.9 53.8 405-429 bp 1.0 49.7
ccsA-ndhD_p3 CAATTCAGCAATTGTGGCGT TCACTATCAGTTGAAAAGGTTGA 45.0 34.8 57.9 55.8 411-435 bp 1.0 49.6
ccsA-ndhD_p4 CAATTCAGCAATTGTGGCGT CACTATCAGTTGAAAAGGTTGAAGT 45.0 36.0 57.9 57.7 410-434 bp 1.0 49.6
ccsA-ndhD_p5 TTCAGCAATTGTGGCGTCTA TCAGTTGAAAAGGTTGAAGT 45.0 35.0 57.8 52.8 402-426 bp 1.0 49.6