Hotspot

rpl32-trnL-UAG

SSC · 113387-114253 · 867 bp

Back to Lysionotus

Pi 0.0167

MarkerSeek score 61.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 867
variable_sites: 64
pi: 0.0167
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAGGGGTATTGTGCATCGT	TTTAGGAGATGACATGCCGC	50.0	50.0	58.0	58.0	781-1082 bp	1.0	88.7
rpl32-trnL-UAG_p2	GAAGGGGTATTGTGCATCGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	726-1027 bp	1.0	88.3
rpl32-trnL-UAG_p3	GAAGGGGTATTGTGCATCGT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1197-1498 bp	1.0	87.7
rpl32-trnL-UAG_p4	GAAGGGGTATTGTGCATCGT	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	1198-1499 bp	1.0	87.7
rpl32-trnL-UAG_p5	GAAGGGGTATTGTGCATCGT	AAAAATAGCACTTGGGCCGA	50.0	45.0	58.0	58.1	1177-1478 bp	0.958333	85.7