Hotspot

ycf4-cemA

LSC · 61739-62599 · 861 bp

Back to Lysionotus

Pi 0.00951

MarkerSeek score 58.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 861
variable_sites: 42
pi: 0.00951
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTGACCCGTACTGATGAG	GAGAGAGAAATCCAACAGGACA	55.0	45.5	58.1	57.5	999-1037 bp	1.0	65.4
ycf4-cemA_p2	CGTTGACCCGTACTGATGAG	TGAGAGAGAAATCCAACAGGAC	55.0	45.5	58.1	57.5	1000-1038 bp	1.0	65.4
ycf4-cemA_p3	GAACAAAAAGCCGCCGAATT	GAGAGAGAAATCCAACAGGACA	45.0	45.5	58.2	57.5	958-996 bp	1.0	64.6
ycf4-cemA_p4	GAACAAAAAGCCGCCGAATT	TGAGAGAGAAATCCAACAGGAC	45.0	45.5	58.2	57.5	959-997 bp	1.0	64.6
ycf4-cemA_p5	CGTTGACCCGTACTGATGAG	TCCAACAGGACAAAAACACT	55.0	40.0	58.1	55.3	989-1027 bp	1.0	64.2