Hotspot

rpoB-trnC-GCA

LSC · 27365-28503 · 1139 bp

Back to Lysionotus

Pi 0.0102

MarkerSeek score 61.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 64
pi: 0.0102
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTGCCTGATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1162-1229 bp	0.958333	85.3
rpoB-trnC-GCA_p2	ATTGCCTGATTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1144-1211 bp	0.958333	83.2
rpoB-trnC-GCA_p3	ATTGCCTGATTTCCATCCCC	TTGTTTGATCAGGCGACACC	50.0	50.0	57.9	58.8	1181-1248 bp	0.958333	79.7
rpoB-trnC-GCA_p4	ATTGCCTGATTTCCATCCCC	GACACCCGGATTTGAACTGG	50.0	55.0	57.9	58.8	1167-1234 bp	0.958333	79.2
rpoB-trnC-GCA_p5	CTGATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1157-1224 bp	0.875	74.3