Hotspot

trnH-GUG-psbA

LSC · 83-402 · 320 bp

Back to Lysionotus

Pi 0.0421

MarkerSeek score 69.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 43
pi: 0.0421
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCGAAGCTCCAACAAATG	55.0	44.4	58.1	52.9	273-386 bp	1.0	52.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCGAAGCTCCAACAAATG	50.0	44.4	57.9	52.9	287-400 bp	1.0	52.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TCGAAGCTCCAACAAATG	50.0	44.4	57.9	52.9	286-399 bp	1.0	52.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TCGAAGCTCCAACAAATG	50.0	44.4	57.9	52.9	288-401 bp	1.0	52.0
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	TCGAAGCTCCAACAAATG	50.0	44.4	57.5	52.9	302-415 bp	1.0	52.0