primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCGAAGCTCCAACAAATG	20	18	55.0	44.4	58.1	52.9	0.0	0.0	7.203208	83	402	273-386	1.000000	52.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TCGAAGCTCCAACAAATG	20	18	50.0	44.4	57.9	52.9	0.0	0.0	7.207570	83	402	287-400	1.000000	52.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TCGAAGCTCCAACAAATG	20	18	50.0	44.4	57.9	52.9	0.0	0.0	7.209077	83	402	286-399	1.000000	52.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TCGAAGCTCCAACAAATG	20	18	50.0	44.4	57.9	52.9	0.0	0.0	7.209077	83	402	288-401	1.000000	52.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CGCATGGTGGATTCACAATC	TCGAAGCTCCAACAAATG	20	18	50.0	44.4	57.5	52.9	6.4	0.0	7.554512	83	402	302-415	1.000000	52.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGATGTCGAGACAAGAGC	TGAAGCCACCCAATCACAAA	20	20	50.0	45.0	58.0	57.9	10.1	0.0	0.095197	6213	7301	1266-1584	1.000000	90.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGATGTCGAGACAAGAGC	TTGAAGCCACCCAATCACAA	20	20	50.0	45.0	58.0	57.9	10.1	0.0	0.095197	6213	7301	1267-1585	1.000000	90.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACGATGTCGAGACAAGAGC	AGCAGCTTGCCAAACAAAAG	20	20	50.0	45.0	58.0	58.1	10.1	0.0	0.071996	6213	7301	1540-1856	1.000000	89.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AACGATGTCGAGACAAGAGC	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	10.1	0.0	0.072122	6213	7301	1545-1861	1.000000	89.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AACGATGTCGAGACAAGAGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	10.1	16.9	0.092129	6213	7301	975-1293	0.791667	81.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8618	9431	855-896	1.000000	59.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8618	9431	854-895	1.000000	59.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8618	9431	858-899	1.000000	58.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8618	9431	857-898	1.000000	58.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8618	9431	856-897	1.000000	58.6
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	TAACGATGCGGGTTCGATTC	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.4	52.4	0.0	0.0	7.005652	10207	10415	256-267	1.000000	49.8
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.4	52.4	0.0	0.0	7.001259	10207	10415	257-268	1.000000	49.7
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.0	52.4	0.0	0.0	6.598602	10207	10415	268-279	1.000000	49.5
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	20	21	55.0	38.1	58.1	52.4	0.0	0.0	6.627438	10207	10415	271-282	1.000000	49.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	20	21	55.0	38.1	57.7	52.4	0.0	0.0	6.902452	10207	10415	272-283	1.000000	49.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTGCCTGATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.174726	27365	28503	1162-1229	0.958333	85.3
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTGCCTGATTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.9	58.4	0.0	14.6	0.448089	27365	28503	1144-1211	0.958333	83.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATTGCCTGATTTCCATCCCC	TTGTTTGATCAGGCGACACC	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.857777	27365	28503	1181-1248	0.958333	79.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTGCCTGATTTCCATCCCC	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	57.9	58.8	0.0	0.7	0.927329	27365	28503	1167-1234	0.958333	79.2
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CTGATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	19	20	57.9	50.0	58.0	58.1	0.0	0.0	1.094688	27365	28503	1157-1224	0.875000	74.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	31533	32228	753-948	1.000000	86.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	31533	32228	732-927	1.000000	85.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	31533	32228	729-924	1.000000	85.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	31533	32228	750-945	1.000000	85.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	GCGTTACTCTACCACTGAGC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.248492	31533	32228	811-811	0.041667	39.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	32301	33636	1490-1533	1.000000	83.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	32301	33636	1493-1536	1.000000	82.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GCTCAGTGGTAGAGTAACGC	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.202426	32301	33636	1495-1538	1.000000	82.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	32301	33636	1480-1523	1.000000	82.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AAGCCCTTTTAGCTCAGTGG	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.319690	32301	33636	1506-1549	1.000000	81.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAAATGGGAATAAGACTTG	GCACGTTGGGTCTTTGAAAC	23	20	39.1	50.0	57.6	58.2	0.0	0.0	3.569345	51524	52584	1142-1241	1.000000	58.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAAATGGGAATAAGACTTG	AGAAGGTCTACGGTTCGAGT	23	20	39.1	50.0	57.6	57.8	0.0	0.0	3.601749	51524	52584	1078-1177	1.000000	58.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCAAAATGGGAATAAGACTTG	AATGCACGTTGGGTCTTTGA	23	20	39.1	45.0	57.6	58.3	0.0	0.0	3.718111	51524	52584	1145-1244	1.000000	57.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAAATGGGAATAAGACTTG	ATGCACGTTGGGTCTTTGAA	23	20	39.1	45.0	57.6	58.3	0.0	0.0	3.718111	51524	52584	1144-1243	1.000000	57.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATGGGAATAAGACT	GCACGTTGGGTCTTTGAAAC	21	20	38.1	50.0	55.4	58.2	0.0	0.0	3.729874	51524	52584	1142-1241	1.000000	57.6
ycf4-cemA_p1	ycf4-cemA	1	CGTTGACCCGTACTGATGAG	GAGAGAGAAATCCAACAGGACA	20	22	55.0	45.5	58.1	57.5	0.0	0.0	2.634214	61739	62599	999-1037	1.000000	65.4
ycf4-cemA_p2	ycf4-cemA	2	CGTTGACCCGTACTGATGAG	TGAGAGAGAAATCCAACAGGAC	20	22	55.0	45.5	58.1	57.5	0.0	0.0	2.634214	61739	62599	1000-1038	1.000000	65.4
ycf4-cemA_p3	ycf4-cemA	3	GAACAAAAAGCCGCCGAATT	GAGAGAGAAATCCAACAGGACA	20	22	45.0	45.5	58.2	57.5	0.0	0.0	2.765255	61739	62599	958-996	1.000000	64.6
ycf4-cemA_p4	ycf4-cemA	4	GAACAAAAAGCCGCCGAATT	TGAGAGAGAAATCCAACAGGAC	20	22	45.0	45.5	58.2	57.5	0.0	0.0	2.765255	61739	62599	959-997	1.000000	64.6
ycf4-cemA_p5	ycf4-cemA	5	CGTTGACCCGTACTGATGAG	TCCAACAGGACAAAAACACT	20	20	55.0	40.0	58.1	55.3	0.0	0.0	2.785741	61739	62599	989-1027	1.000000	64.2
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	70149	70972	976-982	1.000000	77.8
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	70149	70972	975-981	1.000000	77.8
rpl20-rps12_p3	rpl20-rps12	3	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	58.0	58.5	4.9	0.0	0.574654	70149	70972	921-927	0.958333	76.8
rpl20-rps12_p4	rpl20-rps12	4	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	70149	70972	938-944	1.000000	76.4
rpl20-rps12_p5	rpl20-rps12	5	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	58.0	58.7	4.9	9.8	0.764938	70149	70972	884-890	0.958333	75.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAAGGGGTATTGTGCATCGT	TTTAGGAGATGACATGCCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.084828	113387	114253	781-1082	1.000000	88.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAAGGGGTATTGTGCATCGT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.162664	113387	114253	726-1027	1.000000	88.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAAGGGGTATTGTGCATCGT	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.127329	113387	114253	1197-1498	1.000000	87.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GAAGGGGTATTGTGCATCGT	TGAAGTAGCAAAGCCTTGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.128687	113387	114253	1198-1499	1.000000	87.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAAGGGGTATTGTGCATCGT	AAAAATAGCACTTGGGCCGA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.120515	113387	114253	1177-1478	0.958333	85.7
ccsA-ndhD_p1	ccsA-ndhD	1	CAATTCAGCAATTGTGGCGT	TCAGTTGAAAAGGTTGAAGT	20	20	45.0	35.0	57.9	52.8	7.4	0.0	5.230955	115383	115623	405-429	1.000000	49.7
ccsA-ndhD_p2	ccsA-ndhD	2	CAATTCAGCAATTGTGGCGT	TCAGTTGAAAAGGTTGAAGTT	20	21	45.0	33.3	57.9	53.8	7.4	0.0	5.304478	115383	115623	405-429	1.000000	49.7
ccsA-ndhD_p3	ccsA-ndhD	3	CAATTCAGCAATTGTGGCGT	TCACTATCAGTTGAAAAGGTTGA	20	23	45.0	34.8	57.9	55.8	7.4	0.0	5.268322	115383	115623	411-435	1.000000	49.6
ccsA-ndhD_p4	ccsA-ndhD	4	CAATTCAGCAATTGTGGCGT	CACTATCAGTTGAAAAGGTTGAAGT	20	25	45.0	36.0	57.9	57.7	7.4	0.0	5.333210	115383	115623	410-434	1.000000	49.6
ccsA-ndhD_p5	ccsA-ndhD	5	TTCAGCAATTGTGGCGTCTA	TCAGTTGAAAAGGTTGAAGT	20	20	45.0	35.0	57.8	52.8	7.4	0.0	5.339512	115383	115623	402-426	1.000000	49.6
ndhD-psaC_p1	ndhD-psaC	1	TGATTCGTGGTAAAGACAAGA	CCGACCGATTTCTTGAGTGT	21	20	38.1	50.0	54.9	57.9	0.0	0.0	4.169881	117145	117244	193-198	1.000000	54.4
ndhD-psaC_p2	ndhD-psaC	2	TGATTCGTGGTAAAGACAAGA	AGCGATGTGAATCTGCATGT	21	20	38.1	45.0	54.9	58.0	0.0	0.0	4.123699	117145	117244	213-218	1.000000	54.0
ndhD-psaC_p3	ndhD-psaC	3	TGATTCGTGGTAAAGACAAGA	TGTGAATCTGCATGTCCGAC	21	20	38.1	50.0	54.9	58.3	0.0	0.0	4.363955	117145	117244	208-213	1.000000	52.3
ndhD-psaC_p4	ndhD-psaC	4	GGAAAATGATTCGTGGTAAAGACA	CCGACCGATTTCTTGAGTGT	24	20	37.5	50.0	57.8	57.9	0.0	0.0	4.329321	117145	117244	199-204	0.958333	51.2
ndhD-psaC_p5	ndhD-psaC	5	GGAAAATGATTCGTGGTAAAGACA	AGCGATGTGAATCTGCATGT	24	20	37.5	45.0	57.8	58.0	0.0	0.0	4.283139	117145	117244	219-224	0.958333	50.9
rps15-ycf1_p1	rps15-ycf1	1	CAGCAGTCGTTGACGTTTTC	AACAGCAAACAAGTTTCGGC	20	20	50.0	45.0	58.0	58.1	3.2	0.0	0.083945	123407	123787	771-786	1.000000	84.0
rps15-ycf1_p2	rps15-ycf1	2	AGTCGTTGACGTTTTCCCAA	AACAGCAAACAAGTTTCGGC	20	20	45.0	45.0	58.0	58.1	3.2	0.0	0.105760	123407	123787	767-782	1.000000	83.8
rps15-ycf1_p3	rps15-ycf1	3	TCCCAACGCGTTACATAAGG	AACAGCAAACAAGTTTCGGC	20	20	50.0	45.0	58.0	58.1	26.1	0.0	0.091693	123407	123787	1162-1177	1.000000	83.6
rps15-ycf1_p4	rps15-ycf1	4	AACACCATGCATTGATGGGT	AACAGCAAACAAGTTTCGGC	20	20	45.0	45.0	58.1	58.1	8.9	0.0	0.137379	123407	123787	1025-1040	1.000000	83.6
rps15-ycf1_p5	rps15-ycf1	5	CAGCAGTCGTTGACGTTTTC	GCACAAAGTTCTGCCTGTTC	20	20	50.0	50.0	58.0	57.9	3.2	0.0	0.130650	123407	123787	910-916	0.791667	73.3
ycf1_p1	ycf1	1	ATCTCTGTTCGTGCGTTCTC	AAATTCGCGCAAGAAAAGGG	20	20	50.0	45.0	58.0	57.9	0.0	8.7	0.080601	127193	127312	418-418	0.416667	60.6
ycf1_p2	ycf1	2	ATCTCTGTTCGTGCGTTCTC	GTATCCCCCGACCTCTACAA	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.093715	127193	127312	1016-1031	0.416667	58.6
ycf1_p3	ycf1	3	CGGGGTGAACCAAAAAGAGA	AAATTCGCGCAAGAAAAGGG	20	20	50.0	45.0	58.0	57.9	1.2	8.7	0.088914	127193	127312	829-835	0.333333	54.0
ycf1_p4	ycf1	4	TCGGGGTGAACCAAAAAGAG	AAATTCGCGCAAGAAAAGGG	20	20	50.0	45.0	58.0	57.9	1.2	8.7	0.088914	127193	127312	830-836	0.333333	54.0
ycf1_p5	ycf1	5	CGGGGTGAACCAAAAAGAGA	GTATCCCCCGACCTCTACAA	20	20	50.0	55.0	58.0	57.9	1.2	0.0	0.102028	127193	127312	1433-1442	0.333333	53.4