Hotspot

rpl22

LSC · 83494-84042 · 549 bp

Back to Lysimachia

Pi 0.0445

MarkerSeek score 77.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 549
variable_sites: 138
pi: 0.0445
unique_haplotype_count: 55
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	GGGTATCCTGCACTTGGAAG	50.0	55.0	58.0	58.0	961-1042 bp	1.0	91.3
rpl22_p2	TACGTGCAATTCCCTCGATG	GGGTATCCTGCACTTGGAAG	50.0	55.0	58.1	58.0	1111-1192 bp	0.936508	88.6
rpl22_p3	CAAGTCGGAAACCAAGTGGA	TTGGGGGAATTTGCACCTAC	50.0	50.0	58.0	58.1	614-677 bp	0.793651	82.9
rpl22_p4	CAAGTCGGAAACCAAGTGGA	AATTGGGGGAATTTGCACCT	50.0	45.0	58.0	58.0	616-679 bp	0.761905	81.8
rpl22_p5	TACGTGCAATTCCCTCGATG	AATTGGGGGAATTTGCACCT	50.0	45.0	58.1	58.0	766-829 bp	0.698413	79.1