Hotspot

rpl32-trnL-UAG

SSC · 113535-114004 · 470 bp

Back to Lysimachia

Pi 0.0455

MarkerSeek score 77.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 470
variable_sites: 140
pi: 0.0455
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TAGACAGAAGACAGGCCACT	CGCATAACCCAAGACCATCA	50.0	50.0	57.7	58.0	1609-1674 bp	0.571429	72.1
rpl32-trnL-UAG_p2	GACAGAAGACAGGCCACTTT	CGCATAACCCAAGACCATCA	50.0	50.0	57.7	58.0	1607-1672 bp	0.555556	71.8
rpl32-trnL-UAG_p3	GACAGAAGACAGGCCACTTT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	1016-1064 bp	0.555556	71.1
rpl32-trnL-UAG_p4	TAGACAGAAGACAGGCCACT	ACTCAGATTGGAGAGCAGGT	50.0	50.0	57.7	58.1	1589-1633 bp	0.507937	69.4
rpl32-trnL-UAG_p5	GACAGAAGACAGGCCACTTT	ACTCAGATTGGAGAGCAGGT	50.0	50.0	57.7	58.1	1587-1631 bp	0.492063	69.2