Hotspot

ndhF-rpl32

SSC · 112466-113366 · 901 bp

Back to Lysimachia

Pi 0.0426

MarkerSeek score 78.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 901
variable_sites: 260
pi: 0.0426
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGCAACTGCACCGGAAAATA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	2244-2326 bp	0.952381	88.3
ndhF-rpl32_p2	ACAAACGCCTTTTGACAAGC	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	2012-2148 bp	0.777778	81.5
ndhF-rpl32_p3	CAAACGCCTTTTGACAAGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	2011-2147 bp	0.777778	81.5
ndhF-rpl32_p4	TAGCAACTGCACCGGAAAAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	2245-2327 bp	0.634921	75.6
ndhF-rpl32_p5	ATTTAGCAACTGCACCGGAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	2248-2330 bp	0.571429	73.1