Hotspot

ndhC-trnV-UAC

LSC · 50007-51042 · 1036 bp

Back to Lysimachia

Pi 0.0333

MarkerSeek score 75.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1036
variable_sites: 208
pi: 0.0333
unique_haplotype_count: 59
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGCTAATCGGGGCTAAAAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	473-1308 bp	0.984127	89.4
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	464-1299 bp	1.0	89.3
ndhC-trnV-UAC_p3	CTTGGCTAATCGGGGCTAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	475-1310 bp	1.0	87.7
ndhC-trnV-UAC_p4	GGCTAATCGGGGCTAAAACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	472-1307 bp	0.984127	87.0
ndhC-trnV-UAC_p5	CTCTGGTCCTTGGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.8	57.8	483-1318 bp	0.952381	86.7