Hotspot

trnF-GAA-ndhJ

LSC · 47601-48318 · 718 bp

Back to Lysimachia

Pi 0.0397

MarkerSeek score 80.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 158
pi: 0.0397
unique_haplotype_count: 58
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATCCGCGCTTGAAACGTAT	50.0	47.4	58.1	57.9	576-901 bp	1.0	83.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCGCGCTTGAAACGTATC	50.0	50.0	58.1	59.1	576-901 bp	1.0	83.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGCGCTTGAAACGTATCTT	50.0	50.0	58.1	59.3	574-899 bp	1.0	81.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	541-866 bp	0.968254	80.5
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	540-865 bp	0.952381	79.9