Hotspot

psaA-ycf3

LSC · 41393-42070 · 678 bp

Back to Lysimachia

Pi 0.0332

MarkerSeek score 77.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 145
pi: 0.0332
unique_haplotype_count: 52
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGTTGAAGATCACGAGACG	50.0	50.0	57.9	57.7	761-827 bp	1.0	88.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGTTGAAGATCACGAGACGT	50.0	50.0	57.9	57.7	760-826 bp	1.0	88.3
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	TGGTTGAAGATCACGAGACG	50.0	50.0	58.5	57.7	714-780 bp	1.0	85.3
psaA-ycf3_p4	TTGAGAAATGACCCGGTCTG	TGGTTGAAGATCACGAGACG	50.0	50.0	57.5	57.7	781-847 bp	0.968254	84.2
psaA-ycf3_p5	TTGAGAAATGACCCGGTCTG	GGTTGAAGATCACGAGACGT	50.0	50.0	57.5	57.7	780-846 bp	0.968254	84.2