Hotspot

psbM-trnD-GUC

LSC · 28891-30092 · 1202 bp

Back to Lysimachia

Pi 0.0328

MarkerSeek score 78.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1202
variable_sites: 245
pi: 0.0328
unique_haplotype_count: 56
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ACGAGAACGAACAATGCAGT	TCAATTGGTTAGAGCACCGC	45.0	50.0	58.1	58.6	976-1343 bp	1.0	86.5
psbM-trnD-GUC_p2	CACGAGAACGAACAATGCAG	TCAATTGGTTAGAGCACCGC	50.0	50.0	57.8	58.6	977-1344 bp	1.0	85.9
psbM-trnD-GUC_p3	GTAGGCACGAGAACGAACAA	TCAATTGGTTAGAGCACCGC	50.0	50.0	58.2	58.6	982-1349 bp	0.984127	85.2
psbM-trnD-GUC_p4	ACGAGAACGAACAATGCAGT	AATTGGTTAGAGCACCGCC	45.0	52.6	58.1	58.1	974-1341 bp	1.0	81.9
psbM-trnD-GUC_p5	CAGTAGGCACGAGAACGAAC	TCAATTGGTTAGAGCACCGC	55.0	50.0	58.7	58.6	984-1351 bp	0.984127	81.7